MCB140L – 2019 – Research on septins – the dark sheep of the cytoskeletal family

In the advanced cell biology lab class for Cell Biology Majors in the College of Biological Sciences at UC Davis, students learn classic and advanced techniques used by cell biologists to understand how cellular machinery contributes to cell functions. This year students analyzed the behavior of septins under various nutrient conditions. Septins are a conserved cytoskeletal filament that interact closely with cell membranes, and they have been implicated in cell division, neuronal plasticity, cell signaling and targeting of intracellular pathogens to the lysosome for destruction. Despite their very interesting biology, septins are far less studied than their “famous” siblings in the cytoskeletal family – actin and microtubules.

After conducting yeast two hybrid studies on septin subunits and membrane proteins, the 140Lers below are taking a well deserved donut-break. Analyzing data uses lots of glucose!!

MCB140Lers on donut break!


Tanahashi Ryoya Visits from Nara Institute of Science and Technology, Japan

In January, we hosted Ryoya, a Ph.D student from the Nara Institute of Technology in Japan join us for a month long visit. Ryoya is from the Laboratory of Applied Stress and Microbiology at Nara, and is supervised by Dr. Takagi.

Ryoya teaching us about how to drink “brown wine”!










Ryoya helped us apply deconvolution algorithm to our spinning disc confocal data on septins. He learned how to use our experimentally determined point spread function data to reduce background, increase intensity and overall increase resolution of septin structures as observed below. Thanks for your hard work Ryoya and come back and visit any time.